Abstract 15589: Cardiac Remuscularization using 3D Bioprinted Cardiac Patches

Introduction: The Dor procedure, or endoventricular circular patch plasty, was first described in 1985 as a method to restore a dilated left ventricle back to its normal elliptical geometry. This procedure excludes the scarred areas of the septum and ventricular wall, and covers the defect using a circular synthetic patch. We have successfully 3D bioprinted cardiac patches using human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs). The purpose of this study is to evaluate the feasibility of performing the Dor procedure using 3D bioprinted cardiac patches. Methods: hiPSC-CMs were co-cultured with human cardiac fibroblasts (FB) and human umbilical vein endothelial cells (EC) at a CM:FB:EC cell ratio of 70:15:15 to create mixed cell spheroids. These spheroids were then positioned in a stainless steel needle array using a 3D bioprinter. After 3 days, the spheroids fused to form a spontaneously beating, electrically connected cardiac patch, which was removed intact from the needle array. Defe...