Synthesis of novel monomeric and homodimeric cyanine dyes with thioacetyl substituents for nucleic acid detection
2007
Abstract Several novel di-, tri- and tetracationic monomeric and homodimeric monomethine cyanine dyes for nucleic acid detection were synthesized by condensation of 3,4-dihydro-(2 H )-1,3-thiazino[2,3- b ]benzooxazolium bromide and appropriate 1-( ω -bromoalkyl)-4-methylpyridinium or 1-( ω -bromoalkyl)-4-methylquinolinium bromide followed by quaternization with N , N , N ′, N ′-tetramethyl-1,3-propanediamine (TMPDA) or N , N , N , N ′, N ′-pentamethyl-1,3-propanediammonium iodide, or bisquaternization with TMPDA or N , N , N ′, N ′-tetramethyl-1,6-hexanediamine (TMHDA).
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