Expression of APPL1 in gastric carcinomas and its clinical significance

Objective To investigate the expression and significance of adaptor protein containing PH domain, PTB domain, and leucine zipper motif 1 (APPL1) in gastric cancers. Methods Expressions of APPL1 protein and mRNA were detected with immunohistochemistry and reverse transcription-polymerase chain reaction (RT-PCR) in 47 surgical specimens of gastric carcinomas (GC) and 27 normal gastric tissue specimens, respectively. Results The expression rate of APPL1 protein was 59.6%(28/47)in GCs, and 26.1% (6/23) in normal gastric tissues, with a statistically significant difference between two groups (P<0.05). The relative expression of mRNA was 0.821±0.141 in GCs, and 0.731±0.112 in normal gastric tissues, with a statistically significant difference between two groups (P<0.05). Conclusions The expressions of APPL1 protein and mRNA are increased in GCs, with a close relationship between GC and APPL1. Key words: Carrier proteins/ME; Stomach neoplasms/ME