FAST DIFFERENTIATION OF MYCOBACTERIAL SPECIES BY USING PCR-SSCP

Methods Using PCR-SSCP analysis the 16S rDNA of mycobacterial species. In sixty clinical specimen, twenty were positive by using both traditional methods and PCR-SSCP. In these twenty species, eighteen were M. Tuberculosis and two were infected by both mycobacterial species and other than mycobacterium tuberculosis. eve of twenty species which were negative by using traditional methods were positive by using PCR-SSCP. All methods showed negative in twenty control species. All experiments reported results in three days. Using PCR-SSCP, there were different profiles in different mycobacterial species, except that there were similar profiles in mycobacterial and M. bovis BCG. It can be used to differentiate mycobacterial species by using PCR-SSCP.