Evaluación de la técnica modificada de tinción Giemsa en la valoración acrosomal de espermatozoides de mamíferos

It is important to design efficient, practical and easy to perform techniques to assess the acrosomal integrity of the sperm. The objective was to modify the Giemsa staining technique for acrosomal NAR assessment in mammalian sperm. A total of 140 lamellae of spermatozoa from 4 different mammals (bovine, ovine, porcine and human) were evaluated, divided into two groups (70 lamellae per group). The first group was evaluated acrosomal NAR with the classic technique of Giemsa staining and the second, with the modified Giemsa technique. The means of acrosomal NAR and the times of each of the techniques were compared. There was no difference between the means of acrosomal NAR in both techniques (p<0.05); however, while the conventional technique takes 115 minutes, the time of realization of the modified technique was 35 minutes, reducing the time 80 minutes, with better image clarity. In conclusion, the modified staining technique decreases the NAR titration time and shows a better sharpness of the image.