Influence of Priming and Inoculation Dose on the Production of Monoclonal Antibodies in Two Age Groups of BALB/c Mice

ABSTRACT An experiment was set up to assess the influence of some parameters on the production of ascites and monoclonal antibodies against circulating excretory-secretory antigens of Taenia saginata cysticerci in mice. The following parameters were examined: time lapse between priming and the inoculation of hybridoma cells, age and body weight of the mice at the time of inoculation, number of cells injected IP, and the resulting antibody titers of the ascites. In this experiment the method used to prime the mice was the only factor having an influence on the amount of ascites produced. Injection of a higher number of hybridoma cells (2−4 × 106 cells) coincided with higher antibody titers and resulted in an earlier ascites production. The antibody titer of the ascites was increasing with time.