AB0572 REDUCED ENZYMATIC ACTIVITY OF ANTIOXIDANT SYSTEM IN SCLEROSIS.

Background: The antioxidant system is a natural barrier to the pathologic effect of reactive oxygen intermediate (ROI) on the tissues of patients with systemic scleroderma (SSD). It is comprised of enzymatic links, of which the cascade of enzymes like superoxide dismutase (SOD), glutathione peroxidase (GP), glutathione reductase (GR) and others are most important. Autoantibodies to enzymes of the antioxidant system can bring about a reduction in their biochemical activity. Objectives: Studying the effect of production of antibodies to antioxidant system enzymes SOD, GP and GR in patients with SSD using immobilized magnetocontrolable adsorbents. Methods: We observed 40 patients with SSD and 30 apparently healthy individuals. Degree I of activity was established in 15 patients (37,5%), degree II – in 24 patients (60%), degree III – in 1 patient (2,5%). Patients with subacute and acute course of the disease, degree of activity II and III were united in one group due to their small number. Antibodies to SOD, GR and GP were determined in blood serum of patients upon admission and discharge using immobilized antigen forms of enzymes (Gontar I.P., 2001). Commercial preparations served as antigens when determining antibodies to SOD, GR and GP. Results: A study of blood sera from healthy individuals showed SOD antibodies level of 0,06±0,004, GP antibodies – 0,045±0,003, GR antibodies – 0,05±0,01. The total activity of SOD in the group of healthy people was 38,2±1,2 IU; GP – 0,153±0,007, GR – 114,3±3,7. Upon admission to hospital patients showed a reliably reduced activity of SOD and GR (p Conclusion: Production of antibodies to antioxidant system enzymes plays an important role in pathogenesis of SSD involving internal organs and tissues and aggravating the course of the disease on the whole. Disclosure of Interests: None declared