Investigation on the distribution and their effects on reproduction traits of three major genes in Jinhua pigs

The PCR RFLP technique was applied to analyze the distribution of estrogen receptor ( ESR ) gene,follicle stimulating hormone β subunit( FSHβ )gene and prolactin receptor ( PRLR ) gene in three lines of Jinhua pigs.The effect of ESR,FSHβ and PRLR on the reproductive traits was analyzed by the SPSS software.The results showed that:The genotype frequencies of AA,AB,BB for ESR were 0 0237,0 3609,0 6154 for Jinhua pig lineⅠ,0,0 5333,0 4667 for Jinhua pig lineⅡ and 0 2909,0 6,0 1091 for Jinhua pig lineⅢ,respectively;The genotype frequencies of AA,AB,BB for PRLR were 0 2560,0 3929,0 3512 for Jinhua pig lineⅠ,0 2,0 2667,0 5333 for Jinhua pig lineⅡ and 0 1273,0 4909,0 3818 for Jinhua pig lineⅢ,respectively;But only the Jinhua pig lineⅠ had the polymorphism of FSHβ ,and with the low frequency of favorable allele B (0 0120).Significant associations were found for the ESR locus with the number of born alive (NBA) in later parities,the NBA and the total number of born (TNB) of all parities( P 0 05) and the NBA of later parities ( P =0 061) in Jinhua pig lineⅠ.The additive effects of allele B were 0 70 and 0 66 for the NBA in later parities and all parities respectively,and 0 90 and 0 84 for the TNB in later parities and all parities respectively.No significant associations were found for the PRLR locus with the reproductive traits for Jinhua pigs in all parities and significant interactions of ESR and PRLR were found for the TNB and the NBA in all parities ( P 0 05) and for the female birth weight (FBW) in later parities ( P 0 01).