수온에 따른 넙치의 특이 항체 반응

The specific antibody response of olive flounder, Paralichthys olivaceus to different water temperature were tested by enzyme-linked immunosorbent assay (ELISA). In the rearing temperature of 15℃, first anti-bovine serum albumin (BSA) antibody titer was appeared after 14 days of immunization, whereas 24∼48 days post-immunization (PI) resulted maximum antibody titer in all 5 experimental fish with optical density (OD) values 1.94∼3.04. At the end of the experiment (84 days), 0.03∼1.28 OD values were observed. In the rearing temperature of 12∼13℃, first antibody titer was found 28 days PI in 2 out of 5 fish. Three fish shown high OD titer (1.88∼2.68) between 56 and 70 days and OD values of 0.49 to 2.35 were observed at 84 days. However, the anti-BSA antibodies of two fish showed less than 0.8 OD values until 84 days. In the rearing temperature of 10℃, specific antibody appeared at 56 days, maximum antibody titer was observed at 70 days in 2 out of 5 fish (OD values: 1.37∼1.53) and 1.00 to 1.11 OD values were observed at 84 days. Rest 3 fish showed OD values of 0.12 to 0.68 much below to that of other 2 fish, throughout the experimental period. In conclusion, specific antibody response of olive flounder at high temperature was much faster, higher and longer than that at lower temperature.