Spin-coated graphene oxide as a biomaterial for Wharton’s Jelly derived mesenchymal stem cell growth: a preliminary study

ABSTRACTGraphene oxide (GO) is a two-dimensional nanomaterial constructed by networks of oxidized carbon atoms. GO has been extensively studied in cellular applications to enhance proliferation and differentiation of embryonic and adult stem cells. Here, GO substrate was coated on a glass support through spin-coating technique. Wharton’s Jelly mesenchymal stem cell (WJ-MSC) was used in this study and confirmed by cell morphology, immunofluorescence of staining surface CD markers (CD105 and STRO-1), as well as differentiation into adipogenic and osteogenic lineages. In Day-1 of cell culture, WJ-MSC was able to adhere and growth on the GO substrate. Subsequent WJ-MSC cultured on the GO substrate has demonstrated promising cell viability for at least six days and this result is comparable to WJ-MSC grown on glass substrate (without GO). This study implies that GO substrate can be a suitable biomaterial for culturing WJ-MSC, which is an alternative mesenchymal stem cells source.