Analysis of Cannabinoids from Leaves of Ancient Cannabis sativa Found in Yanghai Xinjiang,China

To detect two cannabinoids,delta-9-tetrahydro cannabinol(THC) and cannabidiol(CBD) from the leaves of 2500-year-preserved Cannabis sativa unearthed from Yanghai Tombs,Turpan District in Xinjiang,China,high performance liquid chromatogram(HPLC) analysis showed that the amounts of THC and CBD determined from 26.716 g leaves of 2500-year-preserved C.sativa were 0.2928 mg and 0.2830 mg,which covered 0.110%% and 0.106%% of dried materials,respectively.The authentic samples of THC and CBD were isolated from extant leaves of C.sativa and were elucidated on the basis of spectroscopic analysis.