An in-vitro comparison of four antibacterial agents with and without nicotine and their effects on human gingival fibroblasts.

BACKGROUND To compare anti-bacterial activity of 0.12% Chlorhexidine (CHX), 10% Povidone Iodine (PVD), Vega Oral Care Gel (VEGA) and Antioxidant Gel (AO) on Streptococcus mutans, Streptococcus sanguis, Fusobacterium nucleatum and Porphyromonas gingivalis with and without nicotine and to evaluate their effects on human gingival fibroblasts (HGFs). METHODS S. mutans, S. sanguis, P. gingivalis and F. nucleatum were incubated with serial dilutions (1/4, 1/8, 1/16, 1/32 and 1/64) of anti-bacterial agents in media (with and without nicotine). Minimum inhibitory and minimum bactericidal concentrations (MIC/MBC) were measured, and confocal microscopy was performed.  HGFs were exposed to serial dilutions (1/10, 1/100, 1/1000 and 1/10,000) of antibacterial agents with media. Water-soluble tetrazolium-1 (WST-1) assay and lactate dehydrogenase (LDH) assay were used to assess proliferation and cytotoxicity towards HGFs. RESULTS CHX and PVD significantly inhibited growth of all bacterial species (p 0.05). An increased bacterial growth was seen for all species except P. gingivalis with addition of nicotine. CONCLUSION CHX and PVD demonstrate superior antibacterial properties, but significantly reduce HGF proliferation. AO is bacteriostatic at lower dilutions but is highly toxic to HGFs. VEGA was bacteriostatic and demonstrated no detrimental effects on HGF's. This article is protected by copyright. All rights reserved.