The properties of subtilisin, Novo type, immobilized on porous glass

Studies of the properties of subtilisin, Novo type, immobilized on porous glass with the aid of hexamethylene diisocyanate were carried out. The immobilized proteinase preparation shows optimum activity at a pH value of 10.7 and at a temperature between 60-65 o C. It was stable in a wider range of pH and temperature values than the native subtilisin. The K M values for hemoglobin and BAEE were 9.2×10 -5 [M] and 139×10 -5 [M], respectively. Under relatively non-aqueous conditions, immobilised subtilisin was able to synthesize phenylacetic acid ethyl ester