Novel Assay Assessment of Oxidative Stress Biomarkers in Patients with Keratoconus: Thiol–Disulfide Homeostasis

ABSTRACTPurpose: To investigate systemic oxidative stress by evaluating thiol–disulfide homeostasis using a novel automated homeostasis assay in keratoconus patients.Methods: Twenty-eight patients with keratoconus and 30 control subjects of a similar age and gender were enrolled in the present study. The native thiol, total thiol, and disulfide levels and the disulfide–native thiol, disulfide–total thiol, and native thiol–total thiol ratios were analyzed and compared between the keratoconus and control groups using a novel automatized spectrophotometric assay.Results: The median serum native thiol value was 441.5 (362.6–488.1) in the keratoconus group and 467.8 (401.8–564.6) in the control subjects. The median serum total thiol value was 467.9 (375–580.6) in the keratoconus group and 503.3 (437.7–578.5) in the control subjects. The median serum disulfide value was 20.1 (6.2–46.3) in the keratoconus patients and 16.7 (1.1–20.8) in the controls. There were significant differences between the native thiol, t...