Isolation,purification and identification of protein with cholesterolreducing activity from the supernatant of Lactobacillus reuteri

The cell- free supernatant( CFS) of Lactobacillus reuteri DSM122460 exhibited the cholesterol removal ability and the effective components were predicted as proteins. The active component was purified by ultrafiltration,ammonium sulfate precipitation,and DEAE- Sepharose F.F anion exchange chromatography. The specific activity of purified component was 61.6 U / mg with a purification factor of 32.4. The purified protein was electrophoresis pure determined by SDS- PAGE,and the estimated molecular weight was about 60 ku. The active protein was identified as a hypothetical protein by mass spectrometry and its function remains to be further studied.