Regulation of ANP secretion in insulin-dependent diabetes mellitus and the influence of autonomic neuropathy

Abstract In order to determine the effect of diabetic autonomic neuropathy (DAN) on the atrial natriuretic peptide (ANP) response to dynamic stimuli, we studied the ANP response to 60° head-up and 60° leg-up tilt in diabetic subjects with (DAN + ve, n = 8 ) and without (DAN − ve, n = 8 ) evidence of autonomic neuropathy and seven matched non-diabetic controls. Mean baseline plasma ANP concentrations were similar in all three groups. Head-up tilt was associated with a fall in plasma ANP in all seven healthy controls (21.8 (16.8–30.7) to 16.8 (7.1–29.1), P = 0.06 , mean (range)), seven of the eight DAN − ve (16.9 (6.5–33.7) to 8.5 (3.0–21.1), P = 0.015 ) and all eight DAN + ve subjects (27.3 (8.5–101.5) to 15.4 (1.0–67.6), P = 0.044 ). Leg-up tilt caused a rise in plasma ANP in six of the seven healthy controls (17.6 (7.5–27.9) to 22.4 (15.2–48.1), P = 0.041 ), six of the eight DAN − ve (12.5 (7.8–27.8) to 15.5 (7.3–31.3), P = 0.054 ) and seven of the eight DAN + ve subjects (18.2 (2.8–55.1) to 25.1 (4.5–92.8), P = 0.013 ). There was no significant difference in the fall in plasma ANP during head-up tilt or in the rise in plasma ANP during leg-up tilt between the three groups. We conclude that the regulation of ANP secretion is normal in diabetes mellitus, and is unaffected by the presence of autonomic neuropathy.