Time resolution immunity fluorescence kit for detecting content of beta-hCG as well as preparation method and application thereof

The invention discloses a time resolution immunity fluorescence kit for detecting the content of beta-hCG. The time resolution immunity fluorescence kit comprises a box body and a box cover which is arranged above the box body, wherein a separation layer which is used for placing an elisa plate coated by a beta-hCG monoclonal antibody is arranged in the box body; vessel holes for arranging reagent bottles are formed in the box body, and in the vessel holes, a reagent bottle with analysis solution, a reagent bottle with a cleaning solution, a reagent bottle with an enhanced solution, a reagent bottle with a beta-hCG correction product and a reagent bottle with a labeled anti-alpha-TSH monoclonal antibody are placed. In addition, the invention also discloses a preparation method of the kit and application of the kit in detecting the content of beta-hCG. The kit is high in sensitivity, wide in detection range and good in stability. The time resolution immunity fluorescence kit can be full-automatically operated by utilizing a full-automatic time resolution fluorescent immunity analyzer and is simple and time-saving to operate and high in sensitivity reaching up to 0.01mIU/ml, good in accuracy and free from radiation pollution.