Impact evaluation of frozen storage of Lucilia sericata pupae on the development and prolificacy of descendants of Nasonia vitripennis

Objective The study was conducted to determine the effects of frozen storage of Lucilia sericata pupae on the prolificacy of Nasonia vitripennis.Methods The length of growth stages,sizes of descendants,life span of the females,parasitic and reproductive ability of descendants of N.vitripennis within L.sericata pupae were documented.Results Two groups of N.vitripennis in L.sericata pupae,frozen at-20 ℃ and-70 ℃,respectively,demonstrated a growth period of approximately 13 days,the body length of female descendants about 1.8 mm and males about 1.2 mm,the life span of female descendants about 11 days,suggesting no significant differences when compared to the control group(P0.05).A total of 83% to 90% eclosed N.vitripennis could successfully parasitize natural L.sericata pupae,and 73% to 80% emerged,about 37 from each pupa,with the female to male ratio of about 90%,suggesting no significant differences when compared to the control group(P0.05).Conclusion Frozen storage of L.sericata pupae had no effects on the development and prolificacy of N.vitripennis descendants.