Construction and genotype identification of IL-1R1-/-IL-6-/-gene mice

Objective To construct mice with the knockout of both interleukin 1 receptor 1 (IL-1R1) and interleukin 6 (IL-6) genes. Methods We generated the IL-1R1+ /-IL-6+ /-offspring by crossing the IL-1R1+ /+ mice with IL-6+ /+ mice.DNA was extracted from the ear tissue of mouse offspring fand and subjected to PCR for genotype identification.Mice with IL-1R1-/-IL-6-/-were identified.Mice with the genotype of IL-1R1-/-IL-6-/-were further confirmed by the analysis of Western blotting and enzyme-linked immunosorbent assay(ELISA). Results Two mice with genotype of IL-1R1-/-IL-6-/-were obtained. Western blot and ELISA analysis showed IL-1R1 and IL-6 were not expressed in IL-1R1-/-IL-6-/-mice. Conclusion We have successfully established IL-1R1-/-IL-6-/-gene-knockout mice.This work would provide foundation for the further study of the inflammatory diseases related to IL-1R1 and IL-6. Key words: Interleukin-1receptor1; Interleukin-6; Gene-knockout mice; Genotype identification