The Content of Uric Acid in Semen and Germ Cell Apoptosis

Objective To explore the content of uric acid in semen and germ cell apoptosis in human.Methods Seminal fluid analysis was made according to WHO standard.There were four groups according to different density and motility rate of sperms(normal,20,20～40,40).The content of uric acid in semen was measured by uricase-peroxidase method.Using terminal deoxynucleotidyl transferase(TdT) mediated UTP nick end labeling(TUNEL) and Wright's-Giemsa staining for the detection of germ cell apoptosis.Results In 75 infertile patients,the content of uric acid in semen were(163.37±57.15) μmol/L and germ cell apoptosis rate was(16.38±1.25)%,which was significantly different from fertile group(397.60±52.1)μmol/L,(4.61±1.23)%.Density and motility rate were decline accompany with the content of uric acid,and germ cell apoptosis rate ascend.The latter two were significantly negative related(r=0.93,P0.05).The volume of apoptotic germ cell was decreased;chromatin was compact to form the cell-membrane blebs and apoptotic bodies.Conclusion Low concentration of uric acid can increase the frequency of apoptosis testis and the decline of density of sperm,which can include male infertility.