Abstract 10779: Exosomes Derived From Cardiac Progenitor Cells Attenuate Left Ventricular Dysfunction and Remodeling After Myocardial Infarction via Epigenetic Modulations

Increasing evidence shows that exosomes (Exsm) are being developed as an alternative to cellular therapies because of its important role as mediators via an autocrine/paracrine manner in promoting cell-to-cell communication. However, the effects of Exsm derived from lin-/c-kit+ cardiac progenitor cells (CPC) on myocardial repair and epigenetic gene profile after myocardial infarction (MI) are unknown. To this end, the live mouse CPCs were characterized by FACS. Exsm purified from CPC cultured media by ultracentrifugation were identified by transmission electron microscope, nanoparticle tracking analysis and immunoblotting (Figs. A-B). Mice received vehicle or 80 ug of Exsm 2 days after a 60-min coronary occlusion via intramyocardial injection. At 35 days, compared with vehicle group, Exsm treatment resulted in alleviation of LV dysfunction and remodeling assessed by Echo and an increase in viable myocardial tissue in the risk region, suggesting improvement of myocardial repair (Figs. C-D). Furthermore, at...