Effectiveness of enzymes and inoculants on biological pretreatment of different high dry matter lignocellulosic materials

Abstract The objective was to evaluate the effectiveness of enzymes and inoculants on different high dry matter lignocellulosic materials (forage pea, rice, and wheat straw) by using principal component and multiple factor analysis (MFA) to select the appropriate one for field application. The straws were pretreated with no addition, enzymes, inoculants, and their combination. Fermentation quality, chemical and microbial composition (except dry matter, DM), cell-wall components, and digestibility were significantly affected by pretreatment type with a varied significance level. Factor analysis, which allows easier interpretation by reducing the original variables into a smaller number, explains 85.46% of the total variability of original variables, while the first two factors explain 53.30%. Besides, the results of MFA clearly show that straw types were more important than the biological pretreatment type for high DM lignocellulosic materials. Cell-wall fraction and chemical composition seemed the limiting factors that affect to yield of high-quality straw silages.