Application of LC-DAD Metabolic Fingerprinting in Combination with PCA for Evaluation of Seasonality and Extraction Method on the Chemical Composition of Accessions from Lippia alba (Mill.) N. E. Brown and Biological Activities

Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior, Brasil (CAPES), Finance Code 001. The authors thank the Brazilian funding agencies CNPq, FAPESB and FAPESP (PROEM 2014/50299-5, research grant 2013/01710-1 and Table 2. Inhibition percentage of the activity of the AChE-ICER Sample AChE-ICER inhibition activity / % Sample AChE-ICER inhibition activity / % LA01IS 6.70 ± 0.8 LA01HS 32.7 ± 3.3 LA02IS 14.6 ± 2.4 LA02HS 27.4 ± 0.7 LA24IS 18.0 ± 1.3 LA24HS 30.3 ± 4.1 LA32IS 13.4 ± 0.9 LA32HS 6.70 ± 0.5 LA39IS 17.1 ± 1.7 LA39HS 18.5 ± 4.0 LA54IS 16.1 ± 1.1 LA54HS 24.5 ± 0.7 LA01IW 10.3 ± 4.7 LA01HW 28.4 ± 0.2 LA02IW 7.60 ± 3.8 LA02HW 27.2 ± 0.3 LA24IW 15.0 ± 1.1 LA24HW 24.4 ± 3.2 LA32IW 11.5 ± 1.1 LA32HW 22.2 ± 0.8 LA39IW 23.3 ± 1.6 LA39HW 23.2 ± 1.6 LA54IW 14.4 ± 0.1 LA54HW 29.3 ± 4.5 Galanthaminea 87.4 ± 2.2 aPositive control of acetylcholinesterase (AchE; 100 μM). AChE-ICER: AChE immobilized enzyme reactor; LA01-LA54: leaf samples of L. alba accessions; IS: infusion summer; HS: hydroalcoholic summer; IW: infusion winter; HW: hydroalcoholic winter. PD grant 2014/11640-3) for their financial support and scholarships. A. F. B., E. R. P. F., D. P. B., C. L. C. and P. C. L. N. are grateful to CNPq for research fellowships.