Comparison of Three Holding Solutions for Cooled Storage of Equine Embryos

Equine embryos are traditionally transported in Ham’s F10, a culture medium that requires special handling (bubbling with a specific gas mixture) before it can be used for the conservation of equine embryos. Two media have been tested for the cooled storage of equine embryos (Emcare Holding Solution and Vigro Holding Plus) and appear to be suitable alternatives to Ham’s F10. These media offer the advantage that they are ready-for-use products with a relative long shelf life. Author’s addresses: Equitechnic, Le Mesnil Vicomte, France, peter.daels@ivis.org (Daels); Physiology of Reproduction and Behavior, INRA, 37380 Nouzilly, France (Duchamp); Department of Reproduction, Ecole Veterinaire de Nantes, BP 40706, 44307 Nantes, France (Bruyas, Moussa); Labogena, Domaine de Vilvert, 78352 Jouy-en-Josas, France (Mahla). © 2002 AAEP.