Efferent renal nerve stimulation inhibits the antihypertensive function of the rat renal medulla when studied in a cross-circulation model

The aim of this study was to investigate the effects of renal nerve stimulation on the humoral renal antihypertensive system. An isolated kidney (IK) was perfused at normal or high arterial pressures from a normotensive assay rat by means of a perfusion pump. Perfusion pressure (PP) to the IK was 90 mmHg for a control period of 30 min. In three of five experimental groups PP was then increased to 175 mmHg. In two of the groups the renal nerves were stimulated at 2 (P-175 2Hz ) or 5 Hz (P-175 5Hz ) for 60 min. The remaining group served as a control (P-175 C ). In two groups IK pressure was maintained at 90 mmHg with 5 Hz nerve stimulation (P-90 5HZ ) or without nerve stimulation (P-90 C ). MAP of the assay rat decreased by 22 and 27% (P < 0.001) in the P-175 C and P175 2Hz groups, respectively during the 60 min period of nerve stimulation, but remained stable in P-175 5HZ . Renal blood flow increased in the IK when PP was increased in P-175 C , but did not change significantly in P-175 2Hd or P-175 5Hz . Blood pressure remained constant in the assay rat when the IK was perfused at 90 mmHg. The renal excretory functions of the IK decreased in a frequency dependent manner by 2 and 5 Hz renal nerve stimulation compared with P-175 C . We conclude that 5 Hz renal nerve stimulation inhibits the pressure dependent release of humoral depressor substances from an IK perfused at 175 mmHg, whereas this is not seen when stimulating at 2 Hz. It is suggested that the release of antihypertensive substances from the renal medulla requires an increased renomedullary blood flow.