Effects of chum salmon stanniocalcin,a calcium regulatiug hormone in teleosts and its synthetic N-terminal peptide fragment on bone metabolism

CONTENTS 1. INTRODUCTION / p1 2. MATERIALS AND METHODS / p4 　2.1. Preparation of chum salmon stanniocalcin (STC) / p4 　2.2. Intestinal Ca²⁺influx of Atlantic cod / p4 　2.3. Preparation of peptide fragments of STC / p5 　2.4. Amino acid sequence analysis / p5 　2.5. Cloning of cDNA encoding STC / p6 　2.6. Synthesis of N-terminal peptide fragment of STC (peptide N) / p7 　2.7. Calvarial bone resorption assay / p7 　2.8. Generation of osteoclast-like cells / p8 　2.9. Production of cyclic AMP in osteoblast-like cells / p8 　2.10. Synthesis of collagen in calvarial bone / p9 　2.11. Statistical analysis / p9 3. RESULTS / p10 　3.1. Isolation and characterization of STC / p10 　3.2. Amino acid and cDNA sequence analysis of STC / p11 　3.3. Synthesis of peptide N / p12 　3.4. Effects of STC and peptide N on the calvarial bone resorption / p12 　3.5. Effects of STC and peptide N on generation of osteoclast-like cells / p13 　3.6. Effects of peptide N on the production of cyclic AMP in osteoblast-like cells / p14 　3.7. Effects of peptide N on the synthesis of calvarial bone collagen / p14 4. DISCUSSION / p16 　4.1. Chemical properties of STC / p16 　4.2. Effects of STC and peptide N on the metabolism of mammalian bone / p18 5. SUMMARY / p23 6. REFERENCES / p25