MicroRNAs em pacientes com história familiar de carcinoma epidermóide de cabeça e pescoço

Apesar da maioria dos casos de câncer de cabeca e pescoco ocorrer de maneira esporadica e geralmente estar relacionada a exposicao cronica a alcool e tabaco, a historia familiar e a suscetibilidade tem merecido especial interesse nas ultimas duas decadas. O objetivo do presente estudo foi caracterizar o perfil clinico e epidemiologico de pacientes portadores de câncer de cabeca e pescoco e historia familiar de câncer, avaliar o perfil de expressao dos miRNAs em amostras de sangue periferico de pacientes com carcinoma epidermoide de cabeca e pescoco (CECP) e historia familial de câncer com seus respectivos parentes e checar, a partir da utilizacao de bancos de dados de miRNAs, os principais genes regulados e suas eventuais relacoes com neoplasias. Foram selecionados 74 casos usando o banco de dados do Departamento de Cirurgia de Cabeca e Pescoco e Otorrinolaringologia do Hospital A.C. Camargo, Sao Paulo no periodo de 2003 a 2011. Os criterios utilizados para caracterizar os casos familiais de CECP foram: 1) dois ou mais parentes de primeiro grau acometidos por CECP ou tumores relacionados; 2) Idade de aparecimento do CECP inferior a 45 anos em pelo menos um dos membros da familia; 3) Aparecimento do CECP em qualquer idade em casos com ausencia de exposicao previa ao tabaco e alcool ou qualquer outro fator etiologico conhecido. Os tumores considerados como relacionados ao CECP foram aqueles associados com o consumo de tabaco (pulmao, esofago, estomago, pâncreas, figado, rim, bexiga, utero e medula ossea) ou outros tumores epiteliais como carcinoma colorretal, mama e melanoma. Foram coletadas amostras de sangue periferico dos probandos e, quando possivel, de um de seus parentes acometidos por câncer e realizada a analise de expressao de miRNAs por RT-qPCR. O sitio de tumor mais comum nos probandos foi cavidade oral (42%) seguido de laringe (31.5%)...(AU) Although most cases of head and neck cancer (HNSCC) occur sporadically and generally are related to chronic exposure of alcohol and tobacco, family history and genetic susceptibility have earned special interest in the last two decades.The aims of this study were to characterize the clinical and epidemiological profile of patients with head and neck cancer and family history of cancer, evaluate the expression profile of miRNAs in peripheral blood samples from patients with HNSCC and familial history of cancer with their relatives and check, by using miRNAs databases, the main regulated genes and their possible relationship to cancer. A total of 74 cases were selected through pre-existing databases of the Department of Head and Neck Surgery and Otorhinolaryngology, AC Camargo Hospital, Sao Paulo from 2003 to 2011. The criteria used to characterize the familial cases of HNSCC were: 1) two or more first degree relatives affected by HNSCC or related tumors, 2) Age of onset of HNSCC less than 45 years in at least one of the family members, 3) Appearance of HNSCC at any age if no prior exposure to tobacco and alcohol or any other known etiologic factor. Tumors considered related HNSCC were those related to tobacco consumption (lung, esophagus, stomach, pancreas, liver, kidney, bladder, uterus and bone marrow) or other epithelial tumors such as colorectal carcinoma, breast and melanoma. Then, peripheral blood samples of these patients were collected and, when possible, of one of them relatives affected by cancer. Subsequently, the evaluation of miRNAs expression was did by RT-qPCR. The most common tumor sites of probands were oral cavity, with 31 cases (42%), followed by the larynx, 24 cases (31.5%). Among the 74 families, the number of affected relatives was 171, with 121 of first-degree and 50 of second and third degree. In this group there were 19 different tumor sites and the most common were: head and neck (18.6%), breast (16%), colon (13%), stomach (11%)...(AU)