Allium senescens L. Inhibits Reactive Oxygen Species (ROS) Production and Lipid Accumulation During Differentiation in 3T3-L1 cells (LB408)

Allium vegetables are rich in flavonols and organosulfur compounds, which have anti-inflammatory, anti-oxidant, anti-cancer, prevention of cardiovascular system and tumor inhibitory properties. In Allium vegetables, however, only a few studies have been reported whether the effect on lipid accumulation is involved in the regulation of reactive oxygen species (ROS) production. Thus, Allium senescens L., one of the Allium vegetables, was used to show the effects of ROS production and lipid accumulation during adipogenesis in 3T3-L1 cells. Our results indicated that DPPH (1,1-diphenyl-2-picryl hydrazyl) radical scavenging activity of Allium senescens L. increased in a dose depnedent manner. The effects of Allium senescens L. on ROS production and lipid accumulation in 3T3-L1 cells were evaluated by examining ROS-regulating genes and adipogenic trascription factors at the molecular level. Allium senescens L. inhibited ROS production 21.47% at 100 μg/mL and decreased intracellular lipid accumulation by 46.17% ...