Time Lapse Filming of Cellular Interactions in Organ Culture

A method of time lapse filming which enables the 3-dimensional structure of living tissues to be studied has been extended to observe the behaviour of malignant cells on natural membranes. Two types of virus-transformed fibroblasts and a melanoma cell line showed marked differences in behaviour from the non-malignant cells described in Paper I. The malignant cells tended to form points of anchorage on the surface epithelium of the membranes. At a later stage they penetrated into the epithelial layers and sometimes also into the underlying connective tissue.