A study on K469E polymorphism of ICAM1 and ICAM1 plasma level in patients with coronary heart disease

Objective To study the linkage between K469E polymorphism of intercellular adhension molecule 1 （IC4M1） gene with ICAM1 plasma level and coronary heart disease （CHD） in Han population of China. Methods One hundred and sixty-four controls without CHD and 160 patients with CHD were enrolled in our study. By nested PCR with allele-specific oligonucleotide primers, all patients and controls were genotyped for the ICAM1 polymurphism. And the ICAM1 plasma level was measured by ELISA. Results In the patients with CHD, both K allele frequency and the plasma level of ICAM1 were higher than those in control （ P 〈 0.05）. The individual with K allele had higher plasma level of ICAMI than that without K allele （344.34＋ 128.59 pg/L vs 303.54＋ 108.74 pg/L, P =0.008）. K allele enhanced the risk of CHD （P〈0.01, OR= 2.158, 95%CI：1.250-3.727）. There was the K allele cooperation with smoking in influencing the risk of CHD. Conchtsion There is the polymorphism of ICAM1 K469E gene in Han population of China, and the K allele may be a genetic factor influencing the risk of CHD. Key words: intercellular adhesion molecule 1 ;  gene polymorphism;  coronary heart disease