[Receptor-ligand analysis of killer cell Ig-like receptor in Jiangsu Han population].

AIM: To investigate the distribution of killer cell immunoglobulin-like receptor(KIR) and its specific ligand human leukocyte antigen(HLA) in Jiangsu Han population.METHODS: 173 samples from unrelated healthy individuals of Jiangsu Han population were genotyped and observed for KIR,HLA-Cw and HLA-Bw4 using a SYBR Green I real-time PCR and PCR-SSP method,respectively.The number and type of KIR/HLA pairs inherited in each individual were analyzed.RESULTS: In Jiangsu Han population,all four inhibitory KIR(2DL1,2DL2/3,3DL1 and 3DL2) that recognize the classical HLA class I molecules HLA-A,-B and-C were present in 92% of the study group.Frequencies of 2DL2/HLA-C1,2DL3/HLA-C1,2DL1/HLA-C2 and 3DL1/Bw4 were 0.243,0.971,0.457 and 0.590,respectively;frequencies of 2DS1/HLA-C2 and 2DS2/HLA-C1 were 0.162 and 0.231,respectively.54.3% of the cases expressed KIR2DL1 without HLA-C1,32.9% inherited 3DL1 without HLA-Bw4 and 5.8% expressed HLA-Bw4 without 3DL1.27.7% of the individuals had three iKIR/HLA pairs,26% carried two iKIR/HLA pairs,and 25.4% inherited a single iKIR/HLA pair and no one was deficient in all three iKIR/HLA pairs.CONCLUSION: There was disparity between KIR receptor and HLA ligand in Jiangsu Han population.Inhibitory KIR/HLA pair frequency was higher than stimulatory one.About 1/4 of the study group expressed a single iKIR/HLA pair alone.