IL-1, TNF and IL-6 Release by Wound- inflammatory Cells During the Healing Process in Two Strains of Rats

Publisher Summary This chapter presents a study in which the cellular responses during the course of wound healing in two different inbred rat strains, Albino Oxford (AO) and Dark August (DA), are investigated. The rate of wound closure of full-thickness wounds, the type of wound-infiltrating cells, and the time course of interleukin 1(IL-1), interleukin 6 (IL-6), and tumor necrosis factor (TNF) production by wound-derived cells were measured by using a sponge–matrix model. Two round full-thickness wounds were prepared dorsally in both strains with a sharp, round metal blade of 1 cm diameter, and wound diameters were measured during seven days of the healing process. The study demonstrates that DA and AO rats differ in their healing capabilities. The DA rats showed an accelerated wound-healing course that was accompanied by a different pattern of wound cellular infiltration and significantly higher levels of IL-6 and TNF in supernatants of wound-derived cells compared to AO strain. A higher proportion of infiltrating lymphocytes and higher IL-6 and TNF activities in wound-cell supernatants could be one of the mechanisms that underline the accelerated wound-healing course seen in DA rats.