Studies on the infection and parasitism course of sclerotia of Sclerotinia sclerotiorum by three different mycoparasites

Mycoparasitism is an important process in microcosm of microorganisms. Understanding the mechanisms taking place may allow to effectively improved biocontrol of phytopathogens. A sequence of events during mycoparasitism process by three mycoparasites of different origin and aggressiveness was studied through an optical microscope. Additionally, both germination and entry procedure of mycoparasites on host’s surface were observed through an electron scanning microscope. The development of sclerotia parasitism shows many common features in all three mycoparasites indicating very likely both common course and mechanisms. G21-3 (Gliocladium spp.) is the faster and more destructive mycoparasite followed by T12-9 (Trichoderma spp.) and FD6-15 (Fusarium spp.). In the present study, it was presented in a novel way both the appearance of hyphae of D6-15 isolation (Fusarium spp.) intra-cellularly and also the formation of chlamydospores (intra-cellularly) from G21-3 isolation (Gliocladium spp.). G21-3 germination on the sclerotial surface was completed within 15 h of incubation, and germ tubes will be strayed enough before entering into sclerotium. Appressoria and germ tube branches formation were not observed.