PM2.5-induced DNA Damage in Human Bronchial Epithelial Cells

Objective To study the effect of airborne particulate matter PM2.5 on DNA damage in human bronchial epithelial cells(16-HBE).Methods The human bronchial epithelial cells(16-HBE) were exposed to 8,16,32,64,128μg/ml airborne particulate matter PM2.5 collected from Guangzhou.After 24,48 and 72 h of incubation,the cell survival was measured by MTT assay.DNA damage was detected with Comet assay.Results After 24,48 and 72 h of treatment,compared with the negative control,the cell survival rates of 64,128μg/ml groups decreased significandy(P0.05),and the DNA Olive tail moment of all groups increased significantly(P0.05).Compared with the 24 h group,the cell survival rates of all treated groups in 72 h decreased significantly(P0.05),and the DNA Olive tail moment of all groups in 48 and 72 h increased significandy(P0.05).The regression equation between the DNA Olive tail and dose-time is y=-6.481+0.162 dose+0.210 time (R~2=0.842,P0.05).Conclusion Airborne particulate matter PM2.5 can induce human bronchial epithelial cells(16-HBE) DNA damage in a dose-time dependent manner.