SUMO modification selectively regulates transcriptional activity of peroxisome-proliferator-activated receptor γ in C2C12 myotubes

related protein). De-SUMOylation of PPARγ increased ChIP (chromatin immunoprecipitation) of both a recombinant PPRE (PPAR-response element) and endogenous PPREs of the target genes CD36 and FABP3, but ChIP of the PPRE in the ADRP promoter was not affected by SENP2 overexpression. In conclusion, these results indicate that SENP2 de-SUMOylates PPARγ in myotubes, and de-SUMOylation of PPARγ selectively increases the expression of some PPARγ target genes. tor γ (PPARγ), peroxisome-proliferator-activated receptor-response element (PPRE), small ubiquitin-like modifier (SUMO), small ubiquitin-related modifier 1/sentrin/suppressor of mif two 3 homologue 1-specific peptidase 2 (SENP2), transcriptional regulation.