Остеогенная дифференцировка мезенхимальных стволовых клеток костного мозга человека

This study showed the possibility of using human group AB (IV) serum in the protocol of osteogenic induction of human bone marrow-derived mesenchymal stem cells in vitro. Human bone marrow-derived mesenchymal stem cells were an object of research. The osteogenic differentiation was controlled using the methods of light microscopy, histochemistry (von Kossa and alizarin S red staining) and quantitative RT-PCR. The cultivation of human bone marrow-derived mesenchymal stem cells in the osteoinduction medium with human group AB (IV) serum during 21 days was proved to lead to the differentiation of cells of this type into the osteogenic direction as the evidence of characteristic morphological indications of bone tissue, the formation of ossifications, an increased gene expression of alkaline phosphatase, and osteopontin.