Validation of SPME-GC-FID Method for Determination of Fragrance Allergens in Selected Cosmetic Products

An analytical method for simultaneous determination of 24 legislatively restricted fragrance allergens in various cosmetic products was developed and validated using a combination of solid-phase microextraction and gas chromatography with flame ionization detector. The divinylbenzene-polydimethylsiloxane (DVB-PDMS) fiber was evaluated as the best for extraction of all studied fragrance allergens at 40 °C and 20 min extraction time. All calibration curves showed good linearity in the range of 10−1 to 103 μg mL−1. Good repeatability and inter-day precision was determined for the proposed method. Detection limits for individual allergens ranged from 0.007 to 2.7 μg mL−1. The validated method was used to analyze real cosmetic samples. The obtained results indicated that not all of the analyzed cosmetic samples were labeled in accordance with European Cosmetic Directive.