Relation Between Biofilm Formation and Resistance to Antibacterial Agents of Pseudomonas Aeruginosa Isolated from Different Sources

Pseudomonas aeruginosa ( P. aeruginosa ) is an opportunistic pathogen that is a leading cause of morbidity and mortality in immune-compromised individuals. Moreover, it continues to be a major problem in veterinary practice. Overcoming of P. aeruginosa has become so difficult due to its noticed capacity to resist antibiotics. This capacity is owing to its intrinsic and acquired resistance mechanism to resist most antibiotics. Additionally, adaptive antibiotic resistance of P. aeruginosa is recently characterized mechanism, which includes biofilm- mediated resistance and formation of multidrug presister cells and this is responsible for relapse of infections. The study is carried on 160 samples (110 human samples (50 pus, 60 urine), and 50 animal samples (mastitis milk)). The samples were directly transferred to laboratory where they prepared for bacteriological examination for isolation of P. aeruginosa and determination of antibiotic sensitivity test. Result of this study showed that 11 human samples (6 urine , 5 pus) and 4 mastitis milk samples were positive for P. aeruginosa . By testing positive samples for antibiotic sensitivity test, the result showed the higher sensitivity observed for colistin (100%) for all samples. Meanwhile, all samples show resistance for (SXT, ,MFX, TGC, ETP, FOX ,AUG ,CTX) . The rest of antibiotics (AK, TOB, CN, CIP, LEV, IMP, MEM, ETP, PTZ, CRO, CAZ, SCF, CPZ) were variable in sensitivity between samples. Thus, this work is aimed to highlight on antibiotic resistance of P. aeruginosa in both human and animal.