Cold-insoluble globulin mediates the adhesion of rat liver cells to plastic petri dishes

Abstract Adhesion of rat hepatocytes to plastic culture dishes requires a factor present in normal plasma or serum which tentatively is identified as cold-insoluble globulin since (i) cold-insoluble globulin was the only native plasma protein tested showing cell-adhesion mediating activity, and (ii) plasma from which cold-insoluble globulin selectively had been removed lost its ability to induce cell attachment. Under certain circumstances also asialoceruloplasmin became a potent cell adhesion mediating agent. However, cell attachment mediated by asialoceruloplasmin and cold-insoluble globulin, respectively, was demonstrated to involve separate mechanisms.