Analyzing Binding Data

Measuring the rate and extent of radioligand binding provides information on the number of binding sites, and their affinity and accessibility of these binding sites for various drugs. This unit explains how to design and analyze such experiments. Curr. Protoc. Neurosci. 52:7.5.1-7.5.65. © 2010 by John Wiley & Sons, Inc. Keywords: binding; radioligand; radioligand binding; Scatchard plot; receptor binding; competitive binding curve; IC50; Kd; Bmax; nonlinear regression; curve fitting; fluorescence