Multiomic Analysis of Dried Single-Drop Plasma Sample by Integrated Mass Spectrometry Approach

An easy-to-use and fast approach was developed for integrated proteomic and metabolic profiling from dried single-drop plasma sample. Plasma collection, room temperature storage and sample preparation for both proteins and metabolites were seamlessly integrated in one spintip device. MS-based multiomic profiling by the same nano LC-MS system identified more than 150 proteins and 160 metabolites from 1 μL plasma sample in 6 hours. Further combination with micro flow LC and targeted MS made it a promising approach for fast profiling of molecular biomarkers with high sensitivity and accuracy.