Cas9 gRNA engineering for genome editing, activation and repression

Samira Kiani,Alejandro Chavez,Marcelle Tuttle,Richard N Hall,Raj Chari,Dmitry Ter-Ovanesyan,Jason Qian,Benjamin W. Pruitt,Jacob Beal,Suhani Vora,Joanna Buchthal,Emma J. K. Kowal,Mohammad R. Ebrahimkhani,James J. Collins,Ron Weiss,George M. Church

Cas9 gRNA engineering for genome editing, activation and repression

2015

Samira Kiani
Alejandro Chavez
Marcelle Tuttle
Richard N Hall
Raj Chari
Dmitry Ter-Ovanesyan
Jason Qian
Benjamin W. Pruitt
Jacob Beal
Suhani Vora
Joanna Buchthal
Emma J. K. Kowal
Mohammad R. Ebrahimkhani
James J. Collins
Ron Weiss
George M. Church

The length of a single guide RNA (gRNA) determines the function of Cas9. In this study 20-nt gRNAs allowed nuclease activity and genome editing, whereas 14-nt gRNAs mediated transcriptional activation or repression.

Keywords:

Psychological repression
Molecular biology
Bioinformatics
Cas9
RNA editing
Genome
Nuclease
Genome editing
Guide RNA
Biology
Mutation

Correction
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