한국산 카네이션에 발생하는 바이러스

In the survey of virus diseases of carnations in Korea from 2004 to 2006, 3 viruses of carnation mottle virus (CarMV), carnation latent virus (CLV), carnation necrotic fleck virus (CNFV) were identified from the 575 carnation plants in 67 cultivars, based on shape and size of virus particles, host range, distribution pattern of virus particles and inclusion bodies in the infected cells, serological reaction, and coat protein gene. Viral infection rates were CarMV 66.44%, CLV 6.6%, CNFV 6.78% of the 575 samples surveyed. All CLV and CNFV were doubly infected with CarMV, respectively. The average of carnation plants free from these viruses was 33.56%. Carnations from different areas and propagators varied widely in the incidences of the tested viruses. Coat protein (CP) genes of CarMV-K, CarMV-J, CarMV-C isolated from carnations collected in Korea, Japan and China were amplified using specific oligonucleotide primer by RT-PCR. Sequence analysis of amplified CP gene of CarMV-K showed 99.6% nucleotide similarity to CarMV-J and CarMV-C.