Biosurfactant Production with Various Optimization Parameters

Biosurfactants are amphiphilic compounds produced on living surfaces, mostly on microbial cell surfaces, or sometimes excreted extracellularly. They contain hydrophobic and hydrophilic moieties that reduce surface tension, and interfacial tensions between individual molecules at the surface and the interface respectively. Pseudomonas aeruginosa was grown on the basal medium and inspected for rhamnolipid production. Different carbon sources and various conditions of pH, temperature, agitation conditions and time of incubation were varied to find out optimum growing conditions. The optimum production of 131.2gm/350ml was found on the 12th day from the carbon source glucose at pH 7, temperature 370C, and 150 rpm.