Increased Plasma and Optic Nerve Levels of IL-6, TNF-α, and MIP-2 Following Induction of Ischemic Optic Neuropathy in Mice

Purpose: To investigate levels of proinflammatory cytokines in a mouse model of anterior ischemic optic neuropathy (rAION). Methods: AION was induced in C57/BL6 mice and levels of IL-6, TNF-α, and MIP-2 were measured in plasma by ELISA and in the optic nerves by RT-PCR at predetermined intervals. Results: Plasma: IL-6 levels were elevated immediately after rAION induction and decreased gradually thereafter. TNF-α showed an early peak on day 1 and again from day 21. MIP-2 levels were increased until day 7. Optic nerve: IL-6, TNF-α, and MIP-2 levels increased within a few hours and then decreased gradually. IL-6 had a second peak on day 3. Conclusions: Proinflammatory cytokines may play a role in the pathogenesis of rAION.