OPTIMIZACIÓN DE LA PROLIFERACIÓN IN VITRO DE JOJOBA CON LA APLICACIÓN DEL DISEÑO COMPUESTO CENTRAL ROTABLE E INOCULACIÓN CON RIZOBACTERIAS

Optimization of the in vitro proliferation of jojoba (Simmondsia chinensis (Link) Schn.) by using rotable central composite design and inoculation with rhizobacteria Jojoba (Simmondsia chinensis (Link) Schn.) is a plant wich seeds produce oil of high commercial value used in cosmetology and as lubricant. Experiments were conducted using the rotable central composite design (RCCD) in order to get the optimum of its proliferation in vitro and to evaluate the effect of the plant growth-promoting rhizobacteria PGPR). Nodal segments were cultivated in Murashige & Skoog basal medium with different relations of sucrose (S) and 6- benziladenin (BA), calculated by the RCCD. According to the response surface graph, it was obtained that the optimun number of buds developed as shoots was 5.68, corresponding to 22.3/1.6 S/BA relationship. The biotization with 5 x 10 6 cfu·bud -1 of Azotobacter chroococum increased significantly the number of shoots generated per bud (rate of multiplication). In presence of 0.5 and 1 mg·L -1 of BA, increments of