Genetic Identification and Antimicrobial Activity of Streptomyces sp. Strain Je 1–6 Isolated from Rhizosphere Soil of Juniperus excelsa Bieb

Actinobacteria isolated from poorly explored biotopes are of interest as producers of biologically active compounds. A report is given about the producer of lydicamycin Je 1–6 isolated from the rhizosphere soil of J. excelsa Bieb. collected from the Crimean Peninsula, Ukraine, that demonstrated its antagonistic activity against gram-positive bacteria. Based on the 16S rRNA gene sequence analysis, the strain Je 1–6 was affiliated to the Streptomyces genus. The crude extract from the biomass of this strain showed its activity against gram-positive bacteria, including polyresistant clinical isolates of Staphylococcus sp. The dereplication analysis of the extract of the strain Je 1–6 revealed lydicamycin and its congeners: 30-demethyllydicamycin, 30-demethyl-8-deoxylydicamycin, and 8-deoxylydicamycin. MLSA analysis based on the 16S rRNA gene and five housekeeping genes atpD, gyrB, rpoB, recA, and trpB of the strain Je 1–6 showed a high level of homology with Streptomyces sp. ID38640.