Characterization of a novel, trastuzumab resistant human breast cancer cell line

1. Abstract 2. Introduction 3. Materials and Methods 3.1. Patient clinical history 3.2. Maintaining the B585 primary breast cancer xenografts in SCID mice 3.3. Antibodies 3.4. Conjugation of antibodies with fluorescent dyes 3.5. Immunohistochemistry 3.6. Confocal microscopy 3.7. Chromosomal and array comparative genomic hybridization (CGH) 3.8. Gene expression analysis 3.9. Correlation of aCGH and expression data 3.10. Fluorescence in situ hybridization 4. Results 4.1. Immunohistochemical characterization of the B585 xenograft 4.2. Genetic alterations in the original primary breast tumor and the B585 xenograft 4.3. Fluorescence in situ hybridization using centromere and gene specific probes 4.4. Gene expression signature of the B585 xenograft 5. Discussion 6. Acknowledgement 7. References