[No title available] - PubMed

Objective To study the impact of anticoagulant to the quality of umbilical cord blood (UCB). Methods 6060 cord blood units (CBUs) were classified into five groups, such as 28 ml: (10-29) ml, 28 ml: (30-69) ml, 28 ml: (70-109) ml, 28 ml: (110-150) ml and 28 ml: (>150) ml according to volume ratio of anticoagulant and CBVs. The count of pre-cryopreservation total nucleated cell (pre-TNC), the viability of nucleated cell (VNC), the amount of CFU-GM and the ratio changes of CD34+ were evaluated and analyzed statistically. Results It was found that pre-TNC increased with the growth of volume of CBUs (r=0.9937) under the certain volume of antico-agulant, and the TNC in the minimum UCB volume group was (2.57±0.89)×108; the VNC grew up with the increasing count viability of volume (r=0.9897), and the average viability of the minimum volume group remained over 95%; the CFU-GM climbed up with the increasing of volume (r=0.9024), and the number of CFV-GM in minimum volume group reached to of 89/×105; CD34+% grew up with the increase of volume of CBUs (r=0.9641), and the ratio was (0.30±0.19)% for the minimum volume group. Conclusion In certain volume of anticoagulant in collection-bag, pre-TNC, VNC, CFU-GM and CD34+% are all dropped with the decrease of CBUs volume , however, all above-mentioned indexes in the minimun random group still meet the requirement for clinical administration.