Production of 1,3-propanediol from pure and crude glycerol using a UASB reactor with attached biomass in silicone support

Abstract The 1,3-propanediol (1,3-PDO) yield and productivity from glycerol were studied over a 155-day period. A UASB reactor that also contained silicone support for biomass attachment was used to evaluate the optimal operational conditions and microbiota development. The highest average 1,3-PDO yield was 0.54 and 0.48 mol.mol-gly −1 when reactor pH was 5.0–5.5 and the applied loading rate was 18 and 20 g-gly.L −1 .d −1 using the pure and crude substrate, respectively. The productivity was close to 7.5 g.L −1 .d −1 for both substrates; therefore, the direct use of crude glycerol can be valorized in practice. Clostridium was the predominant genus for 1,3-PDO production and C. pasteurianum was dominant in the biofilm. Using crude glycerol, C. beijerinckii dropped strongly; some Clostridium population was then replaced by Klebsiella pneumoniae and Lactobacillus spp. The good process performance and the advances in the microbiota knowledge are steps forward to obtain a more cost-effective system in practice.